Effects of Electromagnetic Fields (RF EMF)
The role of radiofrequency electromagnetic fields (5G) on neuronal development and neuronal health
Background
Neurodegenerative diseases, e.g., Parkinson’s disease (PD), are increasing worldwide. Experimental studies suggest GSM-modulated non-ionizing radiation (NIR) affect the development of neurodegenerative diseases, demonstrating effects such as decreased neurite outgrowth and decreased synaptic plasticity. Gaps in understanding a possible involvement of NIR in pathways relevant for neurodegeneration remain.
Aims
The goal of this research project is to study effects of NIR-5GFR1 at various stages during brain development in an in vitro model that represents more of the complexity of the human brain than previously applied monolayer cells, namely brain organoids. Besides induced pluripotent stem cells (iPSCs) and wild-type (WT) midbrain brain organoids originating from human iPSCs, a hypothesis-driven approach with genetically engineered stable or inducible KO or KI cells will be applied. The proposed research plan focusses on the following objectives: 1. Determine the role of NIR-5GFR1 in the neuronal phenotype and altering key players related to neuronal development at different developmental stages; and 2. unraveling the mechanism through which NIR-5GFR1 compromises genetically engineered brain organoids making them more susceptible to neurodegeneration. The mechanistic studies on key players in cellular pathways involved in neurodevelopment and neurodegeneration is complemented by epigenetic analyses. Functional assays include measurements on electrical activity and propagation.
Methods
Besides induced pluripotent stem cells (iPSCs) and wild-type (WT) midbrain brain organoids originating from human iPSCs, a hypothesis-driven approach with genetically engineered stable or inducible knock-out (KO) or knock-in (KI) cells will be applied.
Significance
This project will provide insight into possible effects of 5G NR FR1 (<6 GHz) RF-EMF exposure into sensitive times of the brain development in a model system that captures most of the complexity of the human brain. The research will unravel effects of NIR on brain structure, phenotype and function during brain development and neurodegeneration but also improves our mechanistic understanding.
Funding: Research Foundation Mobile Communication ETHZ, Federal Office for the Environment, Berne University Research Foundation
